Difference between revisions of "Part:BBa K3408004"
NJAU-Chappie (Talk | contribs) |
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<partinfo>BBa_K3408004 short</partinfo> | <partinfo>BBa_K3408004 short</partinfo> | ||
| − | It is a protein which could suppress | + | It is a protein which could suppress P<sub>CI</sub> so that our switch couldn't be transcribed without oxygen. It is also under the control of P<sub>nar</sub>. |
We have optimized the CI protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria. | We have optimized the CI protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria. | ||
Revision as of 07:53, 6 October 2020
Optimized CⅠ
It is a protein which could suppress PCI so that our switch couldn't be transcribed without oxygen. It is also under the control of Pnar.
We have optimized the CI protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.
The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).
The plots show the quality of the used codon at the indicated codon position.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]