Difference between revisions of "Part:BBa K3376001"
 
Line 19: Line 19:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K3376001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3376001 SequenceAndFeatures</partinfo>
  

Latest revision as of 07:11, 6 October 2020


GFP-Tr/pSB1C3

The basic part of GFP was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression. The GFPmut1 was assembled with a double terminator.

Expression

GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.

T--Mingdao--ww1.png

Transformation

The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light.

T--Mingdao--ww2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644