Difference between revisions of "Part:BBa K3376014"

 
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<partinfo>BBa_K3376014 short</partinfo>
 
<partinfo>BBa_K3376014 short</partinfo>
  
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This part was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression.  
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This part was modified from GFPmut1 ([https://parts.igem.org/Part:BBa_K1159311 Part:BBa_K1159311]) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression.  
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=== Research ===
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Based on our research, the glucose transporter of ''Salmonella'' has a lower Km compared to human small intestine, ''Staphylococcus'' and ''E. coli'', indicating a higher efficiency for glucose uptake. In our study, we demonstrated glucose absorption ability by overexpressing each of these two systems from ''Salmonella'' in ''E. coli''. Please go to our wiki page ([http://2017.igem.org/Team:Mingdao/Demonstrate#demonstrate-md Mingdao iGEM 2017]) for more information.
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[[File:Mingdaophil1026-2.png|550px|center]]
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=== Cloning ===
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The STM1128 gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3. This part has been sequenced.
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[[File:Mingdaophil1026-3.jpeg|400px|center]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:03, 6 October 2020


Aequorea victoria GFPmut1

This part was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression.

Research

Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake. In our study, we demonstrated glucose absorption ability by overexpressing each of these two systems from Salmonella in E. coli. Please go to our wiki page ([http://2017.igem.org/Team:Mingdao/Demonstrate#demonstrate-md Mingdao iGEM 2017]) for more information.

Mingdaophil1026-2.png


Cloning

The STM1128 gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3. This part has been sequenced.

Mingdaophil1026-3.jpeg




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644