Difference between revisions of "Part:BBa K3610020"
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<partinfo>BBa_K3610020 short</partinfo> | <partinfo>BBa_K3610020 short</partinfo> | ||
| − | This part is a modified version of Part:BBa_K1093016. It has been codon optimized for expression in S. cerevisiae and additionally the split was made at amino acids 158 and 159. | + | This part is a modified version of [[Part:BBa_K1093016]]. It has been codon optimized for expression in S. cerevisiae and additionally the split was made at amino acids 158 and 159. |
===Usage and Biology=== | ===Usage and Biology=== | ||
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mCherry is a monomeric red fluorescent protein that has been used for observing components by fusing the sequence to a protein of interes and observing the mCherry by flourescent spectroscopy and microscopy. The mCherry protein is the basis for the bimolecular fluorescence fusion technique which is used to visualize protein-protein interaction. The mCherry is split into two parts, in our case between amino acids 158 and 159. The two components can be fused to proteins of interest and, upon dimerization, are reconstituted to the fully functional red fluorescent protein (Excitation/Emission wavelengths: 587/610 nm). | mCherry is a monomeric red fluorescent protein that has been used for observing components by fusing the sequence to a protein of interes and observing the mCherry by flourescent spectroscopy and microscopy. The mCherry protein is the basis for the bimolecular fluorescence fusion technique which is used to visualize protein-protein interaction. The mCherry is split into two parts, in our case between amino acids 158 and 159. The two components can be fused to proteins of interest and, upon dimerization, are reconstituted to the fully functional red fluorescent protein (Excitation/Emission wavelengths: 587/610 nm). | ||
| − | This part contains the N-terminal part of the split mCherry system and can be used together with Part: | + | This part contains the N-terminal part of the split mCherry system and can be used together with [[Part:BBa_K3610021]] for investigation of protein-protein interaction in S. cerevisiae. |
In our project, we used this part for visualizing the interaction of leucine-rich repeat receptor-like kinases (LRR-RLKs). We fused the protein to the ectodomains of plant pattern-recognition recpetors which need dimerization to initiate an immune response in plants. | In our project, we used this part for visualizing the interaction of leucine-rich repeat receptor-like kinases (LRR-RLKs). We fused the protein to the ectodomains of plant pattern-recognition recpetors which need dimerization to initiate an immune response in plants. | ||
Revision as of 19:36, 4 October 2020
mCherry N-terminal - codon optimized for S. cerevisiae
This part is a modified version of Part:BBa_K1093016. It has been codon optimized for expression in S. cerevisiae and additionally the split was made at amino acids 158 and 159.
Usage and Biology
mCherry is a monomeric red fluorescent protein that has been used for observing components by fusing the sequence to a protein of interes and observing the mCherry by flourescent spectroscopy and microscopy. The mCherry protein is the basis for the bimolecular fluorescence fusion technique which is used to visualize protein-protein interaction. The mCherry is split into two parts, in our case between amino acids 158 and 159. The two components can be fused to proteins of interest and, upon dimerization, are reconstituted to the fully functional red fluorescent protein (Excitation/Emission wavelengths: 587/610 nm).
This part contains the N-terminal part of the split mCherry system and can be used together with Part:BBa_K3610021 for investigation of protein-protein interaction in S. cerevisiae.
In our project, we used this part for visualizing the interaction of leucine-rich repeat receptor-like kinases (LRR-RLKs). We fused the protein to the ectodomains of plant pattern-recognition recpetors which need dimerization to initiate an immune response in plants.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]