Difference between revisions of "Part:BBa K3453136"

 
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<partinfo>BBa_K3453136 short</partinfo>
 
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This part is an sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) expression cassette under the control of the DmRbcL Toehold Switch 2.3 ([[Part:BBa K3453036|BBa K3453036]]) for sequence-based detection of the rosewood species ''Dalbergia maritima'' var. ''pubescens''.
  
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===Usage and Biology===
 
===Usage and Biology===
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In this composite part sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) was equipped by the synthetic DmRbcL Toehold Switch 2.3 ([[Part:BBa K3453036|BBa K3453036]]) and placed under the control of the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and of the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
  
 
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Revision as of 13:07, 4 October 2020


DmRbcL Toehold Switch 2.3 with sfGFP-LVAtag

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the DmRbcL Toehold Switch 2.3 (BBa K3453036) for sequence-based detection of the rosewood species Dalbergia maritima var. pubescens.

Usage and Biology

In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by the synthetic DmRbcL Toehold Switch 2.3 (BBa K3453036) and placed under the control of the T7 promoter (BBa_K2150031) and of the strong SBa_000587 synthetic terminator (BBa_K3453000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]