Difference between revisions of "Part:BBa K3453132"

 
Line 3: Line 3:
 
<partinfo>BBa_K3453132 short</partinfo>
 
<partinfo>BBa_K3453132 short</partinfo>
  
−
to do
+
This part is an sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) expression cassette under the control of the DmRbcL Toehold Switch 1.2 ([[Part:BBa K3453032|BBa K3453032]]).
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
In this composite part sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) was equipped by the synthetic DmRbcL Toehold Switch 1.2 ([[Part:BBa K3453032|BBa K3453032]]) and placed under the control of the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and of the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
  
 
<!-- -->
 
<!-- -->

Revision as of 10:11, 4 October 2020


DmRbcL Toehold Switch 1.2 with sfGFP-LVAtag

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the DmRbcL Toehold Switch 1.2 (BBa K3453032).

Usage and Biology

In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by the synthetic DmRbcL Toehold Switch 1.2 (BBa K3453032) and placed under the control of the T7 promoter (BBa_K2150031) and of the strong SBa_000587 synthetic terminator (BBa_K3453000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]