Difference between revisions of "Part:BBa K090504:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
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This part generates high expression in gram-positive organisms, and it may cause deleterious effects on the cell if used to express certain protein products.
  
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Because the true functional regions of the genomic fragment used to express GFP in the original vector (PAD43-25) were not known, and the annotation was done using a motif finding algorithm, we left much of the downstream region intact to be safe.
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Because the true functional regions of the genomic fragment used to express GFP in the original vector (PAD43-25) were not known and the annotation was done using a motif finding algorithm, we left much of the downstream region intact to be safe.
  
 
===Source===
 
===Source===

Latest revision as of 01:23, 28 October 2008

Gram-Positive Strong Constitutive Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part generates high expression in gram-positive organisms, and it may cause deleterious effects on the cell if used to express certain protein products.

Because the true functional regions of the genomic fragment used to express GFP in the original vector (PAD43-25) were not known and the annotation was done using a motif finding algorithm, we left much of the downstream region intact to be safe.

Source

We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.

References