Difference between revisions of "Part:BBa K103016"

Revision as of 00:47, 28 October 2008

OmpA_linker_omega_linker

It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit omega ( BBa_K103012)
Fusion partners are connected via Gly-Ser-Gly linker
It's base for creation of hunter fusions in 'hunter and prey' selection system
Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
Interaction between hunter and prey (fused with BBa_K103019) makes cells ampicillin resistant
This part uses our NdeI/SacI/BamHI fusion cloning substandard

| This protein structure was predicted using threading and protein fold prediction and may differ from actual one. |}

Sequence and Features

Assembly Compatibility:

@@ Line 13: / Line 13: @@
 *Interaction between hunter and prey (fused with [[Part:BBa_K103019 | BBa_K103019]]) makes cells ampicillin resistant
 *This part uses our NdeI/SacI/BamHI fusion cloning substandard
+| [[Image:Ompalinkomega.jpg|260px]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
+|}
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 <span class='h3bb'>Sequence and Features</span>