Difference between revisions of "Part:BBa K3610008:Design"
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===Design Notes=== | ===Design Notes=== | ||
Codons that showed a codon frequency of less than 5% were changed into codons preferred by C. reinhardtii. If the preferred codon was interfering with the RFC standards, a codon with a lower codon frequency was chosen. | Codons that showed a codon frequency of less than 5% were changed into codons preferred by C. reinhardtii. If the preferred codon was interfering with the RFC standards, a codon with a lower codon frequency was chosen. | ||
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===Source=== | ===Source=== | ||
Revision as of 18:52, 3 October 2020
EFR ectodomain - codon optimized for C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 82
Illegal NheI site found at 1012
Illegal NheI site found at 1930 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1598
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons that showed a codon frequency of less than 5% were changed into codons preferred by C. reinhardtii. If the preferred codon was interfering with the RFC standards, a codon with a lower codon frequency was chosen.
Source
Ordered from IDT