Difference between revisions of "Part:BBa K3588001"
Line 29: Line 29:
 
k<sub>cat</sub> is 9.4 sec<sup>-1</sup>
 
k<sub>cat</sub> is 9.4 sec<sup>-1</sup>
 
K<sub>M</sub>=1.6 µM<ref>http://dx.doi.org/10.1021/bi026131p</ref>
 
K<sub>M</sub>=1.6 µM<ref>http://dx.doi.org/10.1021/bi026131p</ref>
 +
 +
[[File:CocE Caracterisation.png|none|400px|thumb| ''pH dependence on cocE kinetics and stability.''<ref>scihub.wikicn.top/10.1021/bi026131p</ref>]]
  
  

Revision as of 17:04, 3 October 2020


CocE, Rhodococcus sp. (strain MB1 Bresler)

This part is the CDS of the enzyme Cocaine Esterase (CocE) from Rhodococcus sp. (strain MB1 Bresler). This enzyme hydrolyses cocaine faster than other known cocaine esterase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Function

The enzyme CocE catalyses the breakdown of cocaine into benzoate and ecgonine methyl ester [1]

The enzyme hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants. Also efficiently hydrolyzes cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. Is able to prevent cocaine-induced convulsions and lethality in rat.

Properties

Cocaine Substrate Kinetics

kcat is 7.8 sec-1 KM=0.64 µM[2]

Cocaethylene Substrate Kinetics

kcat is 9.4 sec-1 KM=1.6 µM[3]

pH dependence on cocE kinetics and stability.[4]


  1. ↑ http://dx.doi.org/10.1128/aem.66.3.904-908.2000
  2. ↑ http://dx.doi.org/10.1021/bi026131p
  3. ↑ http://dx.doi.org/10.1021/bi026131p
  4. ↑ scihub.wikicn.top/10.1021/bi026131p