Difference between revisions of "Part:BBa K3453001"

 
 
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This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
 
This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
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It is present in our composite part [[Part:BBa_K3453101|BBa_K3453101]] and allows rapid engineering of reporter constructs using Golden Gate Assembly.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 14:49, 3 October 2020


Golden Gate adapter with BsaI cloning sites

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite part BBa_K3453101 and allows rapid engineering of reporter constructs using Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 2