Difference between revisions of "Part:BBa K2290003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
===Contribution: New documentation from Evry_Paris-Saclay 2020=== | ===Contribution: New documentation from Evry_Paris-Saclay 2020=== | ||
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[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430. | [1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430. | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa K2290003 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa K2290003 parameters</partinfo> | ||
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Revision as of 14:14, 3 October 2020
No part name specified with partinfo tag.
Usage and Biology
Contribution: New documentation from Evry_Paris-Saclay 2020
This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG of 3' end. The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].
[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.
Sequence and Features No part name specified with partinfo tag.