Difference between revisions of "Part:BBa K3349002"
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| − | <b>Figure 1:</b> Homology modelling of | + | <b>Figure 1:</b> Homology modelling of PelC from <i>P. amylolyticus </i>. The protein structure was done using iTASSER [2] and SWISS-Model [3]. The two structures were then aligned and compared. A more in depth analysis can be found on our webpage. |
Revision as of 22:35, 1 October 2020
Pectin Lyase PelC
The PelC Pectin Lyase is originally from Paenabacillus amylolyticus . This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].
For purification purposes, this gene has a C-terminal hexa-histidine tag. For more information on our design and use please see our webpage https://2020.igem.org/Team:Lethbridge_HS.
In the beginning of our project we decided to do homology modelling of our pectin lyase enzymes in order to inform our thermostability modelling.
Figure 1: Homology modelling of PelC from P. amylolyticus . The protein structure was done using iTASSER [2] and SWISS-Model [3]. The two structures were then aligned and compared. A more in depth analysis can be found on our webpage.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 829
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 215