Difference between revisions of "Part:BBa K3349002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was codon optimized for use in <i> E. coli </i>. A C-terminal His tag was added based on previous pectin lyase research [ | + | This part was codon optimized for use in <i> E. coli </i>. A C-terminal His tag was added based on previous pectin lyase research [2]. |
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===Source=== | ===Source=== | ||
Revision as of 22:19, 1 October 2020
Pectin Lyase PelC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 829
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 215
Design Notes
This part was codon optimized for use in E. coli . A C-terminal His tag was added based on previous pectin lyase research [2].
Source
This part was originally taken from the uniprot database. The NCBI sequence ID is WP_123062310.1.
References
1. Mie, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification, and biochemical properties of a thermostable pectinase Bacillus halodurans M29. Journal of Molecular catalysis B: Enzymatic. 94, 77-81