Difference between revisions of "Part:BBa K3349002"

Revision as of 22:14, 1 October 2020

Pectin Lyase PelC

The PelC Pectin Lyase is originally from Paenabacillus amylolyticus . This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].

For purification purposes, this gene has a C-terminal hexa-histidine tag. For more information on our design and use please see our <a href="https://2020.igem.org/Team:Lethbridge_HS">webpage</a>.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 829
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 215

@@ Line 5: / Line 5: @@
 The PelC Pectin Lyase is originally from <i> Paenabacillus amylolyticus </i>. This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].
-For purification purposes, this gene has a C-terminal hexa-histidine tag. For more information on our design and use please see our webpage [https://2020.igem.org/Team:Lethbridge_HS].
+For purification purposes, this gene has a C-terminal hexa-histidine tag. For more information on our design and use please see our <a href="https://2020.igem.org/Team:Lethbridge_HS">webpage</a>.
 <!-- Add more about the biology of this part here