Difference between revisions of "Part:BBa K3610007:Design"

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===Design Notes===
 
===Design Notes===
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The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor
+
The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor.
 +
The signal sequence of the full length receptor was cleaved off out of frame.
  
 
===Source===
 
===Source===

Revision as of 08:35, 1 October 2020


EFR Ectodomain without signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor. The signal sequence of the full length receptor was cleaved off out of frame.

Source

Cyril Zipfel Lab at UZH, Switzerland

References