Difference between revisions of "Part:BBa K3610007:Design"

(Design Notes)
(Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
PCR amplified from the full length receptor sequence.
+
Cyril Zipfel Lab at UZH, Switzerland
  
 
===References===
 
===References===

Revision as of 08:34, 1 October 2020


EFR Ectodomain without signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor

Source

Cyril Zipfel Lab at UZH, Switzerland

References