Difference between revisions of "Part:BBa K3610006:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Lenght needed to allow in-frame translation and the encoded amino acids have to be small ones.
+
The signal peptide was cleaved off out-of frame. For translation this part needs to be expressed with a signal sequence.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 08:21, 1 October 2020


EFR from A. thaliana - without signal sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2683
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2683
    Illegal BglII site found at 2348
    Illegal BglII site found at 2376
    Illegal BglII site found at 2919
    Illegal XhoI site found at 2372
    Illegal XhoI site found at 2962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
    Illegal AgeI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal peptide was cleaved off out-of frame. For translation this part needs to be expressed with a signal sequence.

Source

There is no natural counterpart for this sequence.

References