Difference between revisions of "Part:BBa K3610001"

Revision as of 18:11, 30 September 2020

BAK1 from Arabidopsis thaliana

This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.

Usage and Biology

The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.

Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.

In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence. For characterization of the part see Part:BBa_K3610030

Sequence and Features