Difference between revisions of "Part:BBa K3570006"

Revision as of 09:46, 30 September 2020

DPP1 upstream homologous sequence

DPP1 upstream homologous arm used together with DPP1 downstream (BBa_K3570007). When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 50
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 50
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 50
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 50
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3570006 short</partinfo>
-DPP1 up homologous arm, used together with DPP1 down (BBa_K3570007). When DPP1 up put to 5' of the biobrick together with DPP1 down to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do a homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.
+DPP1 upstream homologous arm used together with DPP1 downstream (BBa_K3570007). When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do a homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.
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