Difference between revisions of "Part:BBa K3349007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The part was codon optimized for use in <i>E. coli</i> . The his tag was placed on the C-terminus based on previous pectin lyase studies [ | + | The part was codon optimized for use in <i>E. coli</i> . The his tag was placed on the C-terminus based on previous pectin lyase studies [4]. |
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===Source=== | ===Source=== | ||
Revision as of 17:30, 29 September 2020
Pectin Lyase PelB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 333
Illegal BamHI site found at 1218 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus based on previous pectin lyase studies [4].
Source
The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.
References
1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from Bacillus haldurans M29.