Difference between revisions of "Part:BBa K3349006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [ | + | The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [4]. |
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| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 17:25, 29 September 2020
Pectin Lyase Pnl
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [4].
Source
Sequence was taken from the Uniprot database for synthesis. The sequence ID from NCBI is KAA8782864.1.
References
1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.
2.Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.
3. Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R. and Schwede, T.(2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296-W303.
4. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81