Difference between revisions of "Part:BBa K3349000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | For building thermostable variants of this Pnl enzyme we first did some homology modelling as the crystal structure has not been determined. | ||
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| + | https://2020.igem.org/wiki/images/c/c0/T--Lethbridge_HS--Pnl_modelling.png | ||
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Revision as of 15:54, 29 September 2020
Pectin Lyase Pnl
This Pectin Lyase is from the organism Paenibacillus amylolyticus . Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. A hexa-histidine tag was added to the C-terminus of the coding sequence for nickel affinity purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]