Difference between revisions of "Part:BBa K103003:Design"

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===Design Notes===
 
===Design Notes===
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[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29 Preparation of ΔA (BBa_K103003)]
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B Domain of Staphylococcal protein A was amplified from vector carrying TapTag sequence (pDRIVE+TapTag) using AL_BXNE (5' TAGAATTCGCGGCCGCTTCTAGAGGGCATATGGGATCCAAAACCGCGGCTCTTGCGCAAC  3') and APSacSpe (5' GGACTAGTAGAGCTCCCGTCTACTTTCGGCGCCTGAGC  3') primers, followed by digestion with EcoRI and BcuI
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===Source===
 
===Source===

Revision as of 20:08, 27 October 2008

B domain of Staphylococcal protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29 Preparation of ΔA (BBa_K103003)]

Source

References