Difference between revisions of "Part:BBa K3610013:Design"

 
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===Source===
 
===Source===
  
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The sequence is a modified version of the sequence of [[Part:BBa_K2485100]] from the iGEM registry  
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The sequence is a modified version of the sequence of [[Part:BBa_K1159001]] from the iGEM registry
  
 
===References===
 
===References===

Revision as of 15:57, 13 September 2020


NanoLuc - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.


Source

The sequence is a modified version of the sequence of Part:BBa_K1159001 from the iGEM registry

References