Difference between revisions of "Part:BBa K143080:Design"
m |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K143080 short</partinfo> | <partinfo>BBa_K143080 short</partinfo> | ||
| + | |||
<partinfo>BBa_K143080 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143080 SequenceAndFeatures</partinfo> | ||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| + | |||
The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers. | The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers. | ||
| − | |||
| Line 14: | Line 14: | ||
The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt. | The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt. | ||
| − | |||
| − | |||
| − | |||
Latest revision as of 18:53, 27 October 2008
P43-gsiB RFP expression construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2052
Illegal AgeI site found at 2164 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, RFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS gsiB were taken from papers.
Source
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-gsiB part that was synthesised by GeneArt.