Difference between revisions of "Part:BBa K143078:Design"
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<partinfo>BBa_K143078 short</partinfo> | <partinfo>BBa_K143078 short</partinfo> | ||
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<partinfo>BBa_K143078 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143078 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers. | The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers. | ||
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The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt. | The 5' and 3' amyE integration sequences were PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt. | ||
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Latest revision as of 18:46, 27 October 2008
P43-spoVG GFP expression construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2143
Design Notes
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter P43 and RBS spoVG were taken from papers.
Source
The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the P43-spoVG part that was synthesised by GeneArt.