Difference between revisions of "Part:BBa K3447000"
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets Cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
 
 
The <i>cvaA</i> and <i>cvaB</i> genes encode components of a dedicated secretion system required for extracellular secretion of colistin. The CvaB protein encoded by the <i>cvaB</i> gene is the central membrane transporter of the bacteriocin V secretion system in <i>Escherichia coli</i>. CvaB, together with CvaA and TolC proteins, direct the secretion of colicin V through the cleavage of the bisglycine leader peptide. The sequence alignment shows that CvaB protein belongs to the ABC superfamily.<br><br>
 
The <i>cvaA</i> and <i>cvaB</i> genes encode components of a dedicated secretion system required for extracellular secretion of colistin. The CvaB protein encoded by the <i>cvaB</i> gene is the central membrane transporter of the bacteriocin V secretion system in <i>Escherichia coli</i>. CvaB, together with CvaA and TolC proteins, direct the secretion of colicin V through the cleavage of the bisglycine leader peptide. The sequence alignment shows that CvaB protein belongs to the ABC superfamily.<br><br>
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===Usage and Biology===
 
===Usage and Biology===
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the <i>Enterobacteriaceae</i> family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
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The <i>cvaA</i> and <i>cvaB</i> genes encode components of a dedicated secretion system required for extracellular secretion of colicin V.
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==<b>Design</b>==
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===Design Notes===
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There is a repetitive sequence between <i>cvi</i> and <i>cvaC</i> gene, and other than that there is no need to pay attention.<br>
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===Source===
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This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
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*https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43597&to=43908&strand=true<br>
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*https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP033091.2?report=genbank&from=43886&to=44122&strand=true<br>
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We ordered the part DNA from a synthesis company.<br>
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<span class='h3bb'><b>Sequence and Features</b></span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3447000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3447002 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3447000 parameters</partinfo>
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<partinfo>BBa_K3447002 parameters</partinfo>
 
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===References===
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1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., <i>Journal of Applied Microbiology</i>, <b>122</b>, 1159-1167.<br>
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2. Colicin V can be produced by lactic acid bacteria.(1999), <i>Letters in Applied Microbiology</i>, <b>29</b>, 37-41.<br>
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3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, <i>Plos One</i>, <b>7(4)</b>, e35382.<br>
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4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), <i>Biochemistry</i>, <b>33</b>, 6911-6917.<br>

Revision as of 05:21, 5 September 2020

xynD, which produces xylanase


The cvaA and cvaB genes encode components of a dedicated secretion system required for extracellular secretion of colistin. The CvaB protein encoded by the cvaB gene is the central membrane transporter of the bacteriocin V secretion system in Escherichia coli. CvaB, together with CvaA and TolC proteins, direct the secretion of colicin V through the cleavage of the bisglycine leader peptide. The sequence alignment shows that CvaB protein belongs to the ABC superfamily.


Usage and Biology

Colicin V is a non-ribosome-encoded antibacterial peptide produced by many E. coli strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of E. coli.

The cvaA and cvaB genes encode components of a dedicated secretion system required for extracellular secretion of colicin V.

Design

Design Notes

There is a repetitive sequence between cvi and cvaC gene, and other than that there is no need to pay attention.

Source

This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.

We ordered the part DNA from a synthesis company.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712



References

1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., Journal of Applied Microbiology, 122, 1159-1167.
2. Colicin V can be produced by lactic acid bacteria.(1999), Letters in Applied Microbiology, 29, 37-41.
3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, Plos One, 7(4), e35382.
4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), Biochemistry, 33, 6911-6917.