Difference between revisions of "Part:BBa K3447000:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
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We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.<br>
+
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
−
This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
+
 
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075<br>
 
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075<br>
 
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164<br>
 
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164<br>

Revision as of 14:44, 4 September 2020


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a repetitive sequence between cvaA and cvaB gene. In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.

Source

We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.

References

1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., Journal of Applied Microbiology, 122, 1159-1167.
2. Colicin V can be produced by lactic acid bacteria.(1999), Letters in Applied Microbiology, 29, 37-41.
3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, Plos One, 7(4), e35382.
4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), Biochemistry, 33, 6911-6917.