Difference between revisions of "Part:BBa K3610003"

 
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<partinfo>BBa_K3610006 short</partinfo>
 
<partinfo>BBa_K3610006 short</partinfo>
  
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The BAK1 cell surface receptor from A. thaliana (BBaK3610004) consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a protein kinase.  
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The BAK1 cell surface receptor from A. thaliana [[BBaK3610004]] consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a protein kinase.  
 
For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
 
For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
 
This part has the is the BAK1 receptor without the kinase domain and without the native signal sequence.
 
This part has the is the BAK1 receptor without the kinase domain and without the native signal sequence.

Revision as of 12:01, 2 September 2020


EFR from A. thaliana - without signal sequence

The BAK1 cell surface receptor from A. thaliana BBaK3610004 consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a protein kinase. For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output. This part has the is the BAK1 receptor without the kinase domain and without the native signal sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2683
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2683
    Illegal BglII site found at 2348
    Illegal BglII site found at 2376
    Illegal BglII site found at 2919
    Illegal XhoI site found at 2372
    Illegal XhoI site found at 2962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2683
    Illegal XbaI site found at 363
    Illegal XbaI site found at 2498
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2337
    Illegal AgeI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]