Difference between revisions of "Part:BBa K143076"

 
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__NOTOC__
 
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<partinfo>BBa_K143076 short</partinfo>
 
<partinfo>BBa_K143076 short</partinfo>
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Long:  AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to RFP proein coding region and the registry double terminator (part <bbpart>BBa_J04650</bbpart>.
 
Long:  AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to RFP proein coding region and the registry double terminator (part <bbpart>BBa_J04650</bbpart>.
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The amyE 3' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 5' amyE integration sequence(<bbpart>BBa_K143001</bbpart>) is cloned onto the 5' end of the construct.
 
The amyE 3' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 5' amyE integration sequence(<bbpart>BBa_K143001</bbpart>) is cloned onto the 5' end of the construct.
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The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.
 
The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143076 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143076 SequenceAndFeatures</partinfo>
  

Revision as of 18:16, 27 October 2008

B. subtilis AmyE locus RFP output promoter and RBS tester


Long: AmyE 3' Integration sequence(BBa_K143002) coupled to RFP proein coding region and the registry double terminator (part BBa_J04650.


The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.


The RFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]