Difference between revisions of "Part:BBa K3458003"

Revision as of 07:27, 17 August 2020

35S Promoter + HQT

This part is the L. japonica. hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) coding region, with a composite 35s promoter element.

Usage and Biology

Because this is a composite of promoter and the coding region for the HQT sequence, it can be expressed by its two components--please see BBa_K3458001 and BBa_K414002.

The flower buds of Lonicera japonica are widely used in Chinese medicine for their anti-inflammatory properties. The reason why L. japonica has potent and significant effects is that it contains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from L. japonica..

[File:HQT_Pathway.png|none|thumb|650px|

Characterization

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 191

@@ Line 9: / Line 9: @@
 ===Usage and Biology===
 '''Because this is a composite of promoter and the coding region for the HQT sequence, it can be expressed by its two components--please see [https://parts.igem.org/Part:BBa_K3458001 BBa_K3458001] and [https://parts.igem.org/Part:BBa_K414002 BBa_K414002].'''
+The flower buds of ''Lonicera japonica'' are widely used in Chinese medicine for their anti-inflammatory properties. The reason why ''L. japonica'' has potent and significant effects is that it contains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from ''L. japonica.''.
+[File:HQT_Pathway.png|none|thumb|650px|
 ===Characterization===