Difference between revisions of "Part:BBa K3458004:Design"

 
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===Design Notes===
 
===Design Notes===
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The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of rice. The promoter GluD-1 cannot drive the HQT express in Oryza sativa L. protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) composite element for comparison with this part.
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===Source===
 
===Source===

Revision as of 03:34, 17 August 2020


GluD-1 Promoter + HQT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225


Design Notes

The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of rice. The promoter GluD-1 cannot drive the HQT express in Oryza sativa L. protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT (BBa_K3458004) composite element for comparison with this part.

Source

A

References