Difference between revisions of "Part:BBa K3458004"

Revision as of 03:28, 17 August 2020

GluD-1 Promoter + HQT

This part is the L. japonica. hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) coding region, with a composite GluD-1 promoter element.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 225

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 <partinfo>BBa_K3458004 short</partinfo>
-A
+This part is the ''L. japonica.'' hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) coding region, with a composite GluD-1 promoter element.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The flower buds of ''Lonicera japonica'' are widely used in Chinese medicine for their anti-inflam�matory properties. The reason why ''L. japonica'' has potent and significant effects is that it con�tains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from ''L. japonica.''
+ [[File:HQT_Pathway.png|none|thumb|650px|'''Fig. 1''' A simplified diagram of the three alternative routes for chlorogenic acid biosynthesis. The product names appear underneath the structures. Enzymes involved in this pathway are: PAL, phenylalanine ammonia lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-hydroxycinnamoyl CoA ligase; HCT, hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyl transferase; C3′H, p-coumaroyl 3′-hydroxylase; HQT, hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase; UGCT, UDP glucose: cinnamate glucosyl transferase; HCGQT, hydroxycinnamoyl D-glucose: quinate hydroxycinnamoyl transferase.]]
+The biosynthetic route of synthesizing CGA from caffeoyl-CoA and quinate using hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) has been reported as the most important way for plants to synthesize CGA. And the result of recent study also showed that tissue distribution of HQT was in accordance with the pattern of CGA content.
+''Oryza sativa L.'' glutelin (GluD-1) is a member of the glutelin family. The expression of GluD-1 gene has unique temporal and spatial characteristics. GluD-1 was predominantly expressed in the inner starchy endosperm beginning about 5 DAF and steadily increased until maturity at 30 DAF. Its spatial specificity is caused by the characteristics of the GluD-1 promoter . Studies have shown that  Only about 0.2 kb of the GluD-1 promoter was suffificient to confer inner starchy endosperm-specifific expression. Since our goal is to increase the content of chlorogenic acid in rice seeds specifically, we chose the 1.2kb GluD-1 promoter.
 ===Characterization===