Difference between revisions of "Part:BBa K3458001:Design"
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In order to have a better expression in ''Oryza sativa L.'', we have carried out codon optimization according to the codon preference of ''Oryza sativa L.''. | In order to have a better expression in ''Oryza sativa L.'', we have carried out codon optimization according to the codon preference of ''Oryza sativa L.''. | ||
* In order to meet the assembly requirements, we removed the illegal restriction site. | * In order to meet the assembly requirements, we removed the illegal restriction site. | ||
| − | * In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) and 35S::HQT ([https://parts.igem.org/Part:BBa_K3458003 BBa_K3458003]). | + | * In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) and 35S::HQT ([https://parts.igem.org/Part:BBa_K3458003 BBa_K3458003]). |
===Source=== | ===Source=== | ||
Revision as of 02:25, 17 August 2020
HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- We synthesized this DNA sample through TIANYI HUIYUAN Company.
In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..
- In order to meet the assembly requirements, we removed the illegal restriction site.
- In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003).