Difference between revisions of "Part:BBa K3458000:Design"
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===Design Notes=== | ===Design Notes=== | ||
A | A | ||
| − | |||
===Source=== | ===Source=== | ||
| − | The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..Then we synthesized it at TIANYI HUIYUAN Company. | + | The HQT gene we used comes from the genome of ''Lonicera japonica'' (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of ''Oryza sativa L''..Then we synthesized it at TIANYI HUIYUAN Company. |
===References=== | ===References=== | ||
| − | Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926. | + | Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in ''Lonicera japonica''[J]. Planta Med., 2010,76(16):1921-1926. |
Latest revision as of 01:49, 15 August 2020
HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A
Source
The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..Then we synthesized it at TIANYI HUIYUAN Company.
References
Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926.