Difference between revisions of "Part:BBa K3458002:Design"

Revision as of 01:48, 15 August 2020

GluD-1 Promoter

Assembly Compatibility:

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 __NOTOC__
-<partinfo>BBa_K3458001 short</partinfo>
+<partinfo>BBa_K3458002 short</partinfo>
-<partinfo>BBa_K3458001 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K3458002 SequenceAndFeatures</partinfo>
 ===Design Notes===
-We synthesized this DNA sample through TIANYI HUIYUAN Company.
-In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..
-In order to meet the assembly requirements, we removed the illegal restriction site.
-In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003). (For more information, please see their registration page)
 ===Source===
-The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard  and optimized the codon according to the codon preference of Oryza sativa L..Then we synthesized it at TIANYI HUIYUAN Company.
 ===References===