Difference between revisions of "Part:BBa K3458000"
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<!-- Add more about the biology of this part here -->
 
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===Usage and Biology===
 
===Usage and Biology===
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'''Because this is just the coding region for the HQT sequence, it is not meant to be expressed on its own. For an expressible HQT biobrick part, please see [https://parts.igem.org/Part:BBa_K3458003 BBa_K3458003 ] and  [https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004 ].'''
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The flower buds of ''Lonicera japonica'' are widely used in Chinese medicine for their anti-inflam�matory properties. The reason why ''L. japonica'' has potent and significant effects is that it con�tains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from ''L. japonica.''.
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[[File:HQT_Pathway.png|none|thumb|650px|'''Fig. 1''' A simplified diagram of the three alterna�tive routes for chlorogenic acid biosynthesis. The product names appear underneath the structures. Enzymes involved in this pathway are: PAL, phenyla�lanine ammonia lyase; C4H, cinnamate 4-hydroxy�lase; 4CL, 4-hydroxycinnamoyl CoA ligase; HCT, hy�droxycinnamoyl CoA shikimate/quinate hydroxycin�namoyl transferase; C3′H, p-coumaroyl 3′-hydroxy�lase; HQT, hydroxycinnamoyl CoA quinate hydroxy�cinnamoyl transferase; UGCT, UDP glucose: cinna�mate glucosyl transferase; HCGQT, hydroxycinna�moyl D-glucose: quinate hydroxycinnamoyl trans�ferase.]]
  
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The biosynthetic route of synthesizing CGA from caffeoyl-CoA and quinate using hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) has been reported as the most important way for plants to synthesize CGA. And the result of recent study also showed that tissue distribution of HQT was in accordance with the pattern of CGA content.
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Our team aims to increase the content of chlorogenic acid in rice to make rice health product effect. In order to increase the expression level of chlorogenic acid in Oryza sativa L, our team optimized the codon of the HQT gene of Lonicera japonica and tested the expression level in the rice expression system.Our work is of significance for fur�ther studies concerned with strengthening the ef�ficacy of Oryza sativa L. by means of improved CGA content. (You can find more related information on our wiki)
  
 
===Characterization===
 
===Characterization===
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===References===
 
===References===
  
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Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in ''Lonicera japonica''[J]. Planta Med., 2010,76(16):1921-1926.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==IMPROVEMENT REFERENCE: BNU-China 2017 ==
 
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The part was linked with a blue chromoprotein amilCP to make it visualized. The new part is : <html><a href='https://parts.igem.org/Part:BBa_K2220028'>BBa_K2220028</a></html>(Group: iGEM17_BNU-China), Designed by: Jiawei Xing.
 

Revision as of 14:00, 14 August 2020

HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)

Hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from L. japonica..（After codon optimized according to the codon preference of Oryza sativa L..）


Usage and Biology

Because this is just the coding region for the HQT sequence, it is not meant to be expressed on its own. For an expressible HQT biobrick part, please see BBa_K3458003 and BBa_K3458004 . The flower buds of Lonicera japonica are widely used in Chinese medicine for their anti-inflam�matory properties. The reason why L. japonica has potent and significant effects is that it con�tains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hy�droxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from L. japonica..

Fig. 1 A simplified diagram of the three alterna�tive routes for chlorogenic acid biosynthesis. The product names appear underneath the structures. Enzymes involved in this pathway are: PAL, phenyla�lanine ammonia lyase; C4H, cinnamate 4-hydroxy�lase; 4CL, 4-hydroxycinnamoyl CoA ligase; HCT, hy�droxycinnamoyl CoA shikimate/quinate hydroxycin�namoyl transferase; C3′H, p-coumaroyl 3′-hydroxy�lase; HQT, hydroxycinnamoyl CoA quinate hydroxy�cinnamoyl transferase; UGCT, UDP glucose: cinna�mate glucosyl transferase; HCGQT, hydroxycinna�moyl D-glucose: quinate hydroxycinnamoyl trans�ferase.

The biosynthetic route of synthesizing CGA from caffeoyl-CoA and quinate using hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) has been reported as the most important way for plants to synthesize CGA. And the result of recent study also showed that tissue distribution of HQT was in accordance with the pattern of CGA content.

Our team aims to increase the content of chlorogenic acid in rice to make rice health product effect. In order to increase the expression level of chlorogenic acid in Oryza sativa L, our team optimized the codon of the HQT gene of Lonicera japonica and tested the expression level in the rice expression system.Our work is of significance for fur�ther studies concerned with strengthening the ef�ficacy of Oryza sativa L. by means of improved CGA content. (You can find more related information on our wiki)

Characterization

References

Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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