Difference between revisions of "Part:BBa K3634011:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The ccdB sequence was taken from BBa_K145151 and found to have an illegal BsaI restriction site at 222bp. The appropriate alteration was made to make the part RFC[1000] compatible: c219g (GTC (Val) to got to go (Val)). As native to E.coli, no codon optimisation step was required.
+
The ccdB sequence was taken from BBa_K145151 and found to have an illegal BsaI restriction site at 222bp. The appropriate alteration was made to make the part RFC[1000] compatible: c219g (GTC (Val) to GTG (Val)). As native to E.coli, no codon optimisation step was required.
  
 
===Source===
 
===Source===

Revision as of 10:53, 6 August 2020


ccdB (BsaI Removed)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ccdB sequence was taken from BBa_K145151 and found to have an illegal BsaI restriction site at 222bp. The appropriate alteration was made to make the part RFC[1000] compatible: c219g (GTC (Val) to GTG (Val)). As native to E.coli, no codon optimisation step was required.

Source

The ccdB toxin is found natively in E.coli and the sequence specified in BBa_K145151 by KULeuven iGEM (2008). This sequence was confirmed against pAG415 GPD-EGFP and pCMV SPORT6ccdB plasmids and in silico site-directed mutagenesis removed a BsaI site so that the part was RFC[1000] standard.

References