Difference between revisions of "Part:BBa K3634009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Schmidl et al. (2014) used both promoter and RBS libraries to produce the system termed CcaSR v2. Two plasmids are used for constitutive expression of ccaS and ccaR as this was found to increase output of sfgfp, measured quantitatively. Promoter and RBS combinations were also selected to maximise PCB production. | + | Schmidl et al. (2014) used both promoter and RBS libraries to produce the system termed CcaSR v2. Two plasmids are used for constitutive expression of ccaS and ccaR as this was found to increase output of sfgfp, measured quantitatively. Promoter and RBS combinations were also selected to maximise PCB production. |
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===Source=== | ===Source=== | ||
Revision as of 18:43, 5 August 2020
ccaS + ho1 + pcyA (Optimised for E.coli)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2449
Illegal NheI site found at 2472 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2126
Illegal BglII site found at 2879
Illegal BamHI site found at 1465 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1497
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Schmidl et al. (2014) used both promoter and RBS libraries to produce the system termed CcaSR v2. Two plasmids are used for constitutive expression of ccaS and ccaR as this was found to increase output of sfgfp, measured quantitatively. Promoter and RBS combinations were also selected to maximise PCB production.
Source
The genomic sequences for all three CDS are IDT codon optimised for E.coli, taken from the native Synechocystis sp. PCC6803. Design of the composite part can be fully attributed to the work of Schmidl S.R., Sheth R.U., Wu A. and Tabor J.J. in the paper 'Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems'. Individual sequences for the parts can be found at BBa_K3634006, BBa_K3634007 and BBa_K3634008.