Difference between revisions of "Part:BBa K3634008:Design"

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===Source===
 
===Source===
  
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The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool allowing for the part to be used at RFC[10] and RFC[1000] standard.
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The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool.
  
 
===References===
 
===References===

Revision as of 15:40, 5 August 2020


Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) (Optimised for E.coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence from BBa_I15009 was taken and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard.

Source

The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool.

References

UTAustin iGEM 2004 - https://parts.igem.org/Part:BBa_I15009

IDT Codon Optimisation Tool - https://eu.idtdna.com/CodonOpt