Difference between revisions of "Part:BBa K165018"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K165018 short</partinfo>
 
<partinfo>BBa_K165018 short</partinfo>
  
The SV40 nuclear localization sequence can be added to any coding region to have it expressed in the nucleus in yeast.  This is useful for building synthetic transcription factors.  In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.
+
The SV40 nuclear localization sequence can be added to any coding region to localize its expression to the nucleus in yeast.  This is useful for building synthetic transcription factors.  In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:31, 27 October 2008

SV40 nuclear localization sequence + ADH1 transcription terminator

The SV40 nuclear localization sequence can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]