Difference between revisions of "Part:BBa K3431007:Design"

 
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===Source===
 
===Source===
  
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This is a synthetic sequence based upon Pardee, K. et al. for detection of miRNA-21. We obtained this sequence through custom gene synthesis of IDT.
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This is a synthetic sequence based upon Pardee, K. et al. for detection of miRNA-31. We obtained this sequence through custom gene synthesis of IDT.
  
 
===References===
 
===References===

Revision as of 14:04, 22 July 2020


zr31 Toehold Switch for miR-31 Detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure their minimal free energy are in an appropriate range (lower than 0 kcal/mole)


Source

This is a synthetic sequence based upon Pardee, K. et al. for detection of miRNA-31. We obtained this sequence through custom gene synthesis of IDT.

References