Difference between revisions of "Part:BBa K3431004:Design"

(→‎References)
m
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure its minimal free energy is in an appropriate range (lower than 0 kcal/mole)
 
We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure its minimal free energy is in an appropriate range (lower than 0 kcal/mole)
−
 
  
  

Latest revision as of 13:15, 21 July 2020


zz21_A Toehold Switch for miR-21 Detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure its minimal free energy is in an appropriate range (lower than 0 kcal/mole)


Source

This is a synthetic sequence based upon Pardee, K. et al. for detection of miRNA-21. We obtained this sequence through custom gene synthesis of IDT.

References

Pardee, K., Green, A. A., Takahashi, M. K., Braff, D., Lambert, G., Lee, J. W., ... & Daringer, N. M. (2016). Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell, 165(5), 1255-1266.