Difference between revisions of "Part:BBa K3515012:Design"
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===References=== | ===References=== | ||
| + | Nie, Y., Li, J., Liu, Y., Zhang, Q. and Ma, Q., 2020. A Visual FRET Immunofluorescent Biosensor for Ratiometric Parathyroid Hormone (1–84) Antigen Point-of-Care Detection. Journal of Fluorescence, pp.1-6. | ||
Latest revision as of 14:31, 16 June 2020
Parathyroid Hormone Receptor with cysteine modification(s) to bind to a biosensor.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 26
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Isolated the N- terminal extracellular domain of PTHR, residues 1-60 for binding to PTH. Kept the one C48 residue in place to bind a corresponding cysteine linker arm (for biosensor immobilization). An intensive BLAST search was done to ensure that the C48 residue did not form a crucial interaction with the ligand.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (V00303.1) along with our own modifications.
References
Nie, Y., Li, J., Liu, Y., Zhang, Q. and Ma, Q., 2020. A Visual FRET Immunofluorescent Biosensor for Ratiometric Parathyroid Hormone (1–84) Antigen Point-of-Care Detection. Journal of Fluorescence, pp.1-6.