Difference between revisions of "Part:BBa K3600002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
* activator: Yap1p | * activator: Yap1p | ||
| − | *YCF1 gene encodes | + | *YCF1 gene encodes encodes a 1,515-amino acid ATP-binding cassette (ABC) protein (ref) |
*The promoter YCF1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to toxic effects (ref). | *The promoter YCF1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to toxic effects (ref). | ||
*The binding site for Yap1p, in the promoter YCF1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html> | *The binding site for Yap1p, in the promoter YCF1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html> | ||
Revision as of 18:36, 14 June 2020
YCF1 promoter part plasmid
This part contains the promoter of the gene YCF1 from S. Cerevisiae.
Usage and Biology
- activator: Yap1p
- YCF1 gene encodes encodes a 1,515-amino acid ATP-binding cassette (ABC) protein (ref)
- The promoter YCF1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to toxic effects (ref).
- The binding site for Yap1p, in the promoter YCF1, is TTACTAA.1
- The yeast toolkit for modular assembly 2 was used to assemble transcriptional units. This promoter was used as a part of type II.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal SpeI site found at 2810
Illegal PstI site found at 2819
Illegal NotI site found at 923
Illegal NotI site found at 2829 - 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2412
Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal BglII site found at 1280
Illegal BglII site found at 2283 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 935
Illegal EcoRI site found at 1665
Illegal XbaI site found at 944
Illegal XbaI site found at 1842
Illegal SpeI site found at 2810
Illegal PstI site found at 2819
Illegal NgoMIV site found at 2767 - 1000COMPATIBLE WITH RFC[1000]