Difference between revisions of "Part:BBa K3600004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
* activator: Yap1p | * activator: Yap1p | ||
| − | *GLR1 gene encodes . | + | * The GLR1 gene encodes glutathione reductase, which catalyses the reduction of the oxidized form of glutathione (GSSG) to Glutathione (GSH).<html><a href="#varela1995"><sup>1</sup></a></html> |
| − | *The promoter GLR1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress. | + | * The promoter GLR1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress. |
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*The binding site for Yap1p, in the promoter GLR1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a> | *The binding site for Yap1p, in the promoter GLR1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a> | ||
Revision as of 18:21, 14 June 2020
GLR1 promoter part plasmid
This part contains the promoter of the gene GLR1 from S. Cerevisiae.
Usage and Biology
- activator: Yap1p
- The GLR1 gene encodes glutathione reductase, which catalyses the reduction of the oxidized form of glutathione (GSSG) to Glutathione (GSH).1
- The promoter GLR1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 778
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 778
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 778
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 624
Illegal BsaI.rc site found at 1145