Difference between revisions of "Part:BBa K143072:Design"

 
m
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K143072 short</partinfo>
 
<partinfo>BBa_K143072 short</partinfo>
 +
  
 
<partinfo>BBa_K143072 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143072 SequenceAndFeatures</partinfo>
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.
 
 
  
  
Line 15: Line 14:
  
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.
 
The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.
 
===References===
 

Latest revision as of 16:50, 27 October 2008

AmyE integratable PoPS generator (P43-spoVG) (with CmR)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-spoVG was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.


Source

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-spoVG that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.