Difference between revisions of "Part:BBa K3600001"

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===Usage and Biology===
 
===Usage and Biology===
 
* activator: Yap1p
 
* activator: Yap1p
*TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.
+
*TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html>
 
*The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
 
*The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
 
*The binding site for Yap1p, in the promoter TXR2, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html>
 
*The binding site for Yap1p, in the promoter TXR2, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html>

Revision as of 15:59, 11 June 2020


TRX2 promoter part plasmid

This part contains the promoter of the gene TRX2 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • TXR2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
  • The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.
  • The binding site for Yap1p, in the promoter TXR2, is TTACTAA.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 568
    Illegal BsaI.rc site found at 1089


Measurements

References

[1] Kuge, S. & Jones, N. YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. The EMBO Journal 13, 655–664 (1994).