Difference between revisions of "Part:BBa K3515010:Design"
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===References=== | ===References=== | ||
| + | Choi, M., Yamada, S. and Makishima, M., 2011. Dynamic and ligand-selective interactions of vitamin D receptor with retinoid X receptor and cofactors in living cells. Molecular pharmacology, 80(6), pp.1147-1155. | ||
Latest revision as of 17:07, 29 May 2020
Vitamin D Receptor Binding Protein with cysteine modification(s) to bind to a biosensor.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 189
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a Cysteine for immobilization (S224C) opposite to the active site region. Removed C34S, C120L, C169V, C201S, C235F, and C242T to ensure only one cysteine would bind to an immobilization linker arm. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (J03258.1) along with our own modifications.
References
Choi, M., Yamada, S. and Makishima, M., 2011. Dynamic and ligand-selective interactions of vitamin D receptor with retinoid X receptor and cofactors in living cells. Molecular pharmacology, 80(6), pp.1147-1155.